Isolation and characterization of influenza virus recombinants with high and low neuraminidase activity
Identifieur interne : 002C09 ( Main/Exploration ); précédent : 002C08; suivant : 002C10Isolation and characterization of influenza virus recombinants with high and low neuraminidase activity
Auteurs : P. Palese [États-Unis] ; J. Schulman [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1974.
English descriptors
- Teeft :
- Activity ratios, Agar, Agar overlay, Allantoic fluids, Amino acids, Antigenic composition, Antiserum, Assay, Clone, Clone cell passage, Clone cells, Crystal violet, Diazonium salt, Dishes inoculated, Eggs inoculated, H3nl antiserum, Hemagglutination inhibition, Hemagglutinin, Heqln2 antiserum, High dilution, Highest dilution, Hon2, Influenza, Influenza virus, Influenza virus neuraminidase, Influenza viruses, Inoculated, Kilbourne, Molecular weight, National institute, Ncuraminidase activity, Neuraminidase, Neuraminidase activity, Neuraminidase band, Neuraminidase content, Neuraminidase inhibition, Nonreducing conditions, Palese, Palese table, Plaque, Plaque inhibition, Plaque size reduction, Polyacrylamide, Protein patterns, Recombinant, Similar differences, Subunit, Terminal dilution, Tissue culture, Total protein, Viral, Viral proteins, Virology, Virus, Virus clones, Virus particles, Virus populations, Wild type virus.
Abstract
Abstract: Two antigenically identical HON2 influenza virus recombinants derived from A/England/42/72 (H3N2) and A/PR8/34 (HON1) were separated from a mixed population by the use of a staining procedure specific for neuraminidase. This procedure employing an artificial neuraminidase substrate (2-(3′-methoxyphenyl)-N-acetylneuraminic acid, MPN) permitted the identification of deeply stained (MPN+) and poorly stained (MPN−) clones of virus in clone 1-5C-4 cells. Upon isolation and purification of the 2 variants, they were found to have eightfold differences in the ratios of neuraminidase/hemagglutination activity. Analysis by polyacrylamide gel electrophoresis demonstrated that the differences in neuraminidase activity reflected differences in the amount of neuraminidase per virion. The MPN− variant which had low neuraminidase content replicated to equal titer in eggs and formed larger plaques than MPN+ variant on clone 1-5C-4 cells. Attempts to isolate clones of MPN− virus from wild type A/England/42/72 virus have not been successful so far.
Url:
DOI: 10.1016/0042-6822(74)90123-8
Affiliations:
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Palese</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Microbiology, Mount Sinai School of Medicine of the City University of New York</wicri:cityArea></affiliation></author><author><name sortKey="Schulman, J" sort="Schulman, J" uniqKey="Schulman J" first="J." last="Schulman">J. Schulman</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Microbiology, Mount Sinai School of Medicine of the City University of New York</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1974">1974</date><biblScope unit="volume">57</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="227">227</biblScope><biblScope unit="page" to="237">237</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Activity ratios</term><term>Agar</term><term>Agar overlay</term><term>Allantoic fluids</term><term>Amino acids</term><term>Antigenic composition</term><term>Antiserum</term><term>Assay</term><term>Clone</term><term>Clone cell passage</term><term>Clone cells</term><term>Crystal violet</term><term>Diazonium salt</term><term>Dishes inoculated</term><term>Eggs inoculated</term><term>H3nl antiserum</term><term>Hemagglutination inhibition</term><term>Hemagglutinin</term><term>Heqln2 antiserum</term><term>High dilution</term><term>Highest dilution</term><term>Hon2</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus neuraminidase</term><term>Influenza viruses</term><term>Inoculated</term><term>Kilbourne</term><term>Molecular weight</term><term>National institute</term><term>Ncuraminidase activity</term><term>Neuraminidase</term><term>Neuraminidase activity</term><term>Neuraminidase band</term><term>Neuraminidase content</term><term>Neuraminidase inhibition</term><term>Nonreducing conditions</term><term>Palese</term><term>Palese table</term><term>Plaque</term><term>Plaque inhibition</term><term>Plaque size reduction</term><term>Polyacrylamide</term><term>Protein patterns</term><term>Recombinant</term><term>Similar differences</term><term>Subunit</term><term>Terminal dilution</term><term>Tissue culture</term><term>Total protein</term><term>Viral</term><term>Viral proteins</term><term>Virology</term><term>Virus</term><term>Virus clones</term><term>Virus particles</term><term>Virus populations</term><term>Wild type virus</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Two antigenically identical HON2 influenza virus recombinants derived from A/England/42/72 (H3N2) and A/PR8/34 (HON1) were separated from a mixed population by the use of a staining procedure specific for neuraminidase. This procedure employing an artificial neuraminidase substrate (2-(3′-methoxyphenyl)-N-acetylneuraminic acid, MPN) permitted the identification of deeply stained (MPN+) and poorly stained (MPN−) clones of virus in clone 1-5C-4 cells. Upon isolation and purification of the 2 variants, they were found to have eightfold differences in the ratios of neuraminidase/hemagglutination activity. Analysis by polyacrylamide gel electrophoresis demonstrated that the differences in neuraminidase activity reflected differences in the amount of neuraminidase per virion. The MPN− variant which had low neuraminidase content replicated to equal titer in eggs and formed larger plaques than MPN+ variant on clone 1-5C-4 cells. Attempts to isolate clones of MPN− virus from wild type A/England/42/72 virus have not been successful so far.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Palese, P" sort="Palese, P" uniqKey="Palese P" first="P." last="Palese">P. Palese</name></region><name sortKey="Schulman, J" sort="Schulman, J" uniqKey="Schulman J" first="J." last="Schulman">J. Schulman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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